Expression of Cytochrome1b1in Human Endometrial Cancer ...

[Abstract]

Objective:Endometrial cancer is one of three gynecologic malignanttumors, including cervical cancer and ovarian cancer. In recent years, themorbidity is increasing and it seriously endangers the health of women. But itspathogenesis is not yet clear. It is believed that exposure to endogenous orexogenous estrogens, which is not opposed by progesterone, increases the riskof developing endometrial cancer. Estrogen related intracellular metabolismincludes cytochrome P4501B1(CYP1B1)-mediated oxidation (mainly for thehydroxylation) and catechol-O-methyltransferase (COMT)-mediatedmethylation. Cytochrome P4501B1(CYP1B1) is involved in estrogenmetabolism? whose metabolites are associated with the initiation andprogression of cancer. catechol-O-methyltransferase (COMT) convertsestrogen metabolites to nontoxic substances and cleans them out of body. Theaim of the study was to detect CYP1B1, COMT cell localization in cell andexpression difference between endometrial cancer and normal endometrium,then to explore the significance of both in endometrial cancer progression,then to provide a new idea to dometrial cancer etiology, diagnosis andtreatment.Methods:Total40Endometrial cancer tissues were collected aftersurgery and were confirmed by pathology in the department of Gynecologyand Obstetrics in the4th hospital of Hebei Medical Universiry.Total35normal endometrium tissues were chosen in the same area as control group(from patients of cervical carcinoma, without uterine fibroids, endometrialhyperplasia, ovarian cancer and other related gynecological diseases). Alltissues were stored in10%formalin, were made into wax block after7days.Immunohistochemistry (SP) was used to detect the expression of CYP1B1andCOMT. The Olympus DP70imaging system was used to take pictures. Stained specimens was analysised by image analysis system Image-ProPlus5.1(USA). Statistical analysis was performed using SPSS13.0softwarepackage. Comparison of two groups was performed by t-test and three groupsby the One-Way ANOVA. Comparison of two groups, with uneven variance,was performed by the non-parametric test. P<0.05was considered significantfor all statistical analysis.Results:1CYP1B1and COMT were expressed in cell plasma, few in theinterstitial and (or) glandular cell nuclear. This study was mainly to comparethe expression in the cytoplasm of glandular epithelial cell.2The age difference was not significant between the endometrial cancerpatients and healthy controls.The distribution of CYP1B1and COMTexpressions in the case and control groups were normal.3The mean optical density (OD) of CYP1B1in the case group?0.259?0.044? was significantly higher than that in control group?0.169?0.184?, the difference was statistically significant (P=0.000);While the OD of COMT in the case group ?0.208?0.058? was lower thanthat in control group, the difference was statistically significant (P=0.000).4The expression of CYP1B1were significantly different in differentstages of endometrial carcinoma (P<0.05), and the more advanced stage, thestronger the expression was. In the case group, CYP1B1expression in ERpositive group was stronger than in ER negative group. The expression ofCOMT in P53positive group was stronger than in P53negative group (P=0.035).5The expresstion of CYP1B1and COMT had positive linear correlationin the control group(r=0.384, P=0.025).Conclusions:1The expression of CYP1B1in endometrial cancer tissues was higherthan that in the normal tissues, indicating that CYP1B1may play an importantrole in the occurrence of endometrial cancer, which provide a new way ofdiagnosis, treatment and prognosis about endometrail cancer. 2With ER expression increased gradually, CYP1B1expression showeda rising trend, suggesting that CYP1B1may be regulated through ER pathway.3The expression of CYP1B1in endometrial cancer tissues was strongerthan that in the normal tissues, suggesting that CYP1B1may be used as theprognosis indicator of endometrial cancer.4The expression of COMT in control group was higher than that in thecase group, implying that COMT may play a protective role in endometrialcancer occurrence and development.5The expresstion of CYP1B1and COMT had positive linear correlationin the control group, indicating that both coordinates to maintain the normalestrogen metabolism, and to prevent excessive production of carcinogens.

Title: Expression of Cytochrome1b1in Human Endometrial Cancer Associated with ER Expression

Category: Liver Cancer

Filename: Expression of Cytochrome1b1in Human Endometrial Cancer Associated with ER Expression.pdf

Pages: 145

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